In Vitro Cytotoxicity Evaluation of a Novel Root
Repair Material
Hui-min Zhou, PhD,*† Ya Shen, DDS, PhD,† Zhe-jun Wang, DDS, PhD,†‡ Li Li, PhD,* Yu-feng Zheng, PhD,*§ Lari Ha€kkinen, DDS, PhD,jj and Markus Haapasalo, DDS, PhD†
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Repair Material
Hui-min Zhou, PhD,*† Ya Shen, DDS, PhD,† Zhe-jun Wang, DDS, PhD,†‡ Li Li, PhD,* Yu-feng Zheng, PhD,*§ Lari Ha€kkinen, DDS, PhD,jj and Markus Haapasalo, DDS, PhD†
Abstract
Introduction: This study examined the effect of a new bioactive dentin substitute material (Biodentine) on the viability of human gingival fibroblasts.
Methods: Bio- dentine, White ProRoot mineral trioxide aggregate (MTA), and glass ionomer cement were evaluated. Human gingival fibroblasts were incubated for 1, 3, and 7 days both in the extracts from immersion of set materials in culture medium and directly on the surface of the set materials immersed in culture medium. Fibro- blasts cultured in Dulbecco modified Eagle medium were used as a control group. Cytotoxicity was evaluated by flow cytometry, and the adhesion of human gingival fibroblasts to the surface of the set materials was as- sessed by using scanning electron microscopy. The data of cell cytotoxicity were analyzed statistically by using a one-way analysis of variance test at a signifi- cance level of P < .05.
Results: Cells exposed to extracts from Biodentine and MTA showed the highest viabilities at all extract concentrations, whereas cells exposed to glass ionomer cement extracts displayed the lowest viabilities (P < .05). There was no significant difference in cell viabilities between Biodentine and MTA during the entire experimental period (P > .05). Human gingival fibroblasts in contact with Biodentine and MTA attached to and spread over the material surface after an overnight culture and increased in numbers after 3 and 7 days of culture.
Conclusions: Biodentine caused gingival fibroblast reaction similar to that by MTA. Both materials were less cytotoxic than glass ionomer cement. (J Endod 2013;39:478–483)
Key Words
Biocompatibility, Biodentine, calcium silicate–based
materials, cell adhesion, cytotoxicity, flow cytometry,
glass ionomer, human gingival fibroblast, MTAIntroduction: This study examined the effect of a new bioactive dentin substitute material (Biodentine) on the viability of human gingival fibroblasts.
Methods: Bio- dentine, White ProRoot mineral trioxide aggregate (MTA), and glass ionomer cement were evaluated. Human gingival fibroblasts were incubated for 1, 3, and 7 days both in the extracts from immersion of set materials in culture medium and directly on the surface of the set materials immersed in culture medium. Fibro- blasts cultured in Dulbecco modified Eagle medium were used as a control group. Cytotoxicity was evaluated by flow cytometry, and the adhesion of human gingival fibroblasts to the surface of the set materials was as- sessed by using scanning electron microscopy. The data of cell cytotoxicity were analyzed statistically by using a one-way analysis of variance test at a signifi- cance level of P < .05.
Results: Cells exposed to extracts from Biodentine and MTA showed the highest viabilities at all extract concentrations, whereas cells exposed to glass ionomer cement extracts displayed the lowest viabilities (P < .05). There was no significant difference in cell viabilities between Biodentine and MTA during the entire experimental period (P > .05). Human gingival fibroblasts in contact with Biodentine and MTA attached to and spread over the material surface after an overnight culture and increased in numbers after 3 and 7 days of culture.
Conclusions: Biodentine caused gingival fibroblast reaction similar to that by MTA. Both materials were less cytotoxic than glass ionomer cement. (J Endod 2013;39:478–483)
Key Words
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