Effectiveness of three instrumentation systems to remove Enterococcus faecalis from root canals
M. Matos Neto, S. S. F. Santos, M. V. P. Lea ̃o, S. M. Habitante, J. R. D. D. Rodrigues & A. O. C. Jorge
Abstract
Matos Neto M, Santos SSF, Lea ̃o MVP, Habitante SM,
Rodrigues JRDD, Jorge AOC. Effectiveness of three instru-
mentation systems to remove Enterococcus faecalis from root
canals. International Endodontic Journal, 45, 435–438, 2012.
Aim
To assess the effectiveness of three systems of
mechanical preparation to reduce Enterococcus faecalis
within root canals.
Methodology
Twenty-four human single-rooted
canine teeth were standardized to a length of 17 mm
and the canal contents removed using a size 20 K-file,
as the last apical file. After irrigation and sterilization,
the canals were contaminated with E. faecalis and
incubated for 21 days at 37 °C with 5% CO2. Then, the
teeth were divided into three groups for mechanical
preparation with: ProTaper rotary system, ProTaper
manual system and manual K-files. Samples of the root
canal contents, before and after the debridement, were collected with sterile paper points for 1 min. Then, the
samples were diluted and plated in Brain Heart Infusion
(BHI) agar. The colony-forming units were counted and
the percentage reduction calculated. The reduction and
log CFU mL)1 were compared between groups using
Wilcoxon nonparametric test and two-way analysis of
variance, respectively.
Results
There was a significant reduction in the
number of CFU/mL (P = 0.000) before and after
debridement for all the systems used. However, there
was no significant difference between the systems.
Conclusion
All the three instrumentation systems
reduced E. faecalis counts to a similar degree.
Keywords: debridement, Enterococcus faecalis, Pro- Taper.
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